Manchester City were 2-0 up against West Ham at the London Stadium and thought they had a third in the second half, only for Mike Dean to rule Gabriel Jesus' goal out for an offside infringement by Raheem Sterling.
Later in the game West Ham were on the receiving end of VAR however when Sergio Aguero was ordered to retake his missed penalty after encroachment from West Ham's Declan Rice - the Argentinian scored at the second time of asking.
Much of the backlash against VAR in this game appeared to be prompted by the fact Sterling was offside by a matter of millimetres, leading some to question whether the laws of the game needed to be changed.
Three goals were ruled out in total by VAR and over 70 checks were made on decisions over the weekend. It will no doubt take some time to adapt.
We take a look at some of the reaction to its introduction...
The technology had a high-profile supporter, with former Liverpool defender Jamie Carragher:
Sterling shoulders the burden of first call:
Former England forward Stan Collymore was irritated by some fan reaction meanwhile, tweeting:
Former England midfielder Danny Murphy wants VAR to be scrapped.
"Something doesn't feel right at the moment when we're watching football," Murphy told talkSPORT.
"I know the majority of what VAR did at the weekend was good - corrected some decisions that would not have been - but if you gave me the choice I'd knock VAR on the head.
"I agree with the Wolves manager (Nuno Espirito Santo). Two minutes celebrating, then you take away that joy. Then the other fans are celebrating a non-goal."
Murphy was also critical of City having a goal disallowed: "Do we really want to see goals disallowed for an offside that's an armpit ahead of another armpit by four millimetres?
"He might be offside by four millimetres by the letter of the law. But it's an entertainment business, isn't it?
"The Sterling goal that was allowed later, there was muted celebrations.
"You could see he was thinking 'Is this going to get taken away?' and the Man City fans were clapping. It was like applause at the end of the ballet!"
Match of the Day presenter Gary Lineker described himself as "pro VAR" on social media: